In the Autosegmental-Metrical model, the prosodic structure is defined as a hierarchy of Accent Phrases (AP). Groups of AP form intermediate prosodic phrases ip, which in turn are grouped into Intonation Phrases IP, and finally sequences of IP form the sentence intonation unit. In this hierarchy several constraints affect the prosodic structure, such as the AP 7 syllables rule, the stress clash conditions, eurhythmicity and syntactic clash. These constraints have been established essentially from read sentences data. They lead to an experimental justification in the observed synchronization of AP’s syllabic chunking by Delta brain waves. This paper investigates the validity of the prosodic structure constraints on spontaneous speech data in French, as well as the adequacy of the Delta waves characteristics to synchronize AP data.