The measurement of Quality of Experience is largely assessed using the Mean Opinion Score (MOS) that has been defined in telecommunication domain. It is known that MOS measurement focuses on system influencing factors, and strives to filter-out human, contextual and content factors. However, the emerging conceptualization of QoE gives attention to non-functional factors. These factors are known to play a central role in user experience and still largely unexplored in context of Quality of Experience (QoE) and User Experience (User Experience). This paper analyses the impact of human emotion and video content on the measured QoE. The emotional state of subjects is evaluated after and before each visualized video sequence. A statistical analysis is realized in order to identify the importance of emotion state and video content type in shaping QoE.