Intervocalic voicing neutralization in Tohoku Japanese was studied based on auditory impressions until our previous research revealed this phenomenon by acoustically and quantitatively measuring voice onset time (VOT). While the research excluded fully pre-voiced tokens due to measurement difficulties, the current study included such tokens by using the method proposed in an earlier study. We also measured the onset F0 of the following vowel and preceding vowel duration to discuss the possibility of secondary cues for the voicing contrast. Our result confirmed the overlap in VOT values in the positive region, which agrees with our previous result, and revealed another overlap in the negative region from the fully pre-voiced tokens newly added in the current study. Moreover, a positive linear correlation between VOT and F0, known as consonant-intrinsic F0, was observed in Tohoku Japanese. Although our results did not support F0 of the following vowel and preceding vowel duration as a secondary cue, investigating acoustic cues may contribute to further increasing our understanding of Tohoku Japanese.