The Swedish cattle call singing style ‘kulning’ is surprisingly understudied, despite its mythical status in folklore. While some acoustic and physiological aspects have been addressed previously [1,2], a more detailed analysis is still lacking. Previous work [2] showed that sound pressure level (SPL) in kulning tapered off less than in head register as a function of distance, which warrants a study of underlying physiological mechanisms responsible for this. In the present paper, the same singer, singing the same song — in kulning and in head register (“falsetto”) mode — was recorded indoors. Electroglottographic (EGG), stroboscopic, high-speed endoscopic and audio registrations were made. Analyses examined differences between kulning and head register. Results show somewhat higher SPL in kulning than in head register confirming the previous findings. EGG showed longer relative glottal closed time and higher amplitude of the signal in kulning. This suggests better vocal fold contact in kulning. Flexible nasofiberoscopy and high-speed recordings during kulning showed medial and antero-posterior narrowing of the laryngeal inlet, a clear approximation of the false vocal folds and marked adduction of the vocal folds.