Intensive study regarding phonological units in Japanese has been focused upon for the past few years. Numerous researchers have proposed some phonological units, among which bi-moraic foot is a valid candidate, considering various phonological phenomena of the Japanese language, and thus far, it has been thought to play important roles as a phonological basic unit in Japanese ([2] [3][6][7][8]). This paper reports further evidence for bi-moraic foot in Japanese given by explicit segmentation experiments, following Otake (1992,1993) ([4] [5]). In the earlier research, the segmentations at the second mora alone could be construed as evidence for bi-moraic foot in Japanese ([6]). In this paper, it is discussed that not only the segmentations at the second mora but also the ones at the fourth mora can be evidence for bi-moraic foot. This is based upon the idea that if the second moraic segmentations are to be evidence for bi-moraic foot, then the fourth moraic segmentations are multiples of the second moraic segmentations. Hence, the fourth moraic segmentations can be interpreted as binary bi-moraic foot, which support the bi-moraic foot theory in Japanese.